• Gene Ontology annotations (GAF 2.0) on Mycobacterium tuberculosis H37Rv (MYCTU)

• paper-centric: nearly all papers from Pubmed search on MYCTU up to and including publication date 2010 (<PMID 21000000)

• private, one-man effort

• updated yearly

• excluding results based on microarrays only

Quality control

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• several manual checks, incl. random rechecks

• using homegrown ruby scripts, several further tests

• using filter-gene-association.pl script from gene-ontology.org

• applied corrections after check by UniProtKB-GOA group

Statistics

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• 1,166 papers

• 2,455 proteins

• 61 per cent of genome

• 7,739 annotations using 941K

Methods and software

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• Pubmed for title/abstract search of relevant papers among >25,000 MYCTU papers

• Phenote editor for annotations

• Bibdesk bibliography software

• web sites from GO, QuickGO, UniProt, tuberculist.epfl.ch, ChEBI

• added/corrected several GO entries via Sourceforge

• ruby for quality control and translation to GAF 2.0

• perl for quality control